Composite

Part:BBa_K3753017:Design

Designed by: Yiqing Zhang   Group: iGEM21_NJTech_China   (2021-10-16)


UASTEF-pTEF-GFP-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587
    Illegal BsaI.rc site found at 2305


Design Notes

The UASTEF and the promoter of tef2 was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The green fluorescent protein and the terminator CYC1(GFP-CYC1) was obtained by PCR amplification using pRS415-GFP-CYC1 plasmid as template.



Source

Saccharomyces cerevisiae

References